Urocanase of Pseudomonas putida

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Photoactivation of urocanase in Pseudomonas putida. Role of sulfite in enzyme modification.

Urocanase is inactivated in vivo and is reactivated by ultraviolet radiation. The chemistry of this photoregulation of urocanase was studied. Purified enzyme was inactivated by 25 pM sulfite (tl,z, 15 min). Dialyzed sulfite-treated inactive enzyme was photoactivatable. The activity can be cycled repeatedly by alternate ultraviolet light and sulfite treatments. Active enzyme binds 5 times more [...

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Activation of urocanase from Pseudomonas putida by electronically excited triplet species.

Urocanase from Pseudomonas putida becomes inactive in growing and resting cells and, as shown previously, is activated by the direct absorption of ultraviolet light. In this study, we describe the activation of urocanase by energy transfer from triplet indole-3-aldehyde, generated in the peroxidase-catalyzed aerobic oxidation of indole-3-acetic acid. The activation was time-, temperature-, and ...

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Pathogenic significance of Pseudomonas fluorescens and Pseudomonas putida.

Among the members of the genus Pseudomonas, P. aeruginosa and P. pseudomallei were, until recently, considered the human pathogens. However, since the early 1960s, other Pseudomonas species have been found in clinical specimens, and their number and frequency of isolation seem to be growing.' The "simple fluorescent" species, P. fluorescens and P. putida, belong to those that were thought by ea...

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Polynucleotide phosphorylase-deficient mutants of Pseudomonas putida.

In bacteria, polynucleotide phosphorylase (PNPase) is one of the main exonucleolytic activities involved in RNA turnover and is widely conserved. In spite of this, PNPase does not seem to be essential for growth if the organisms are not subjected to special conditions, such as low temperature. We identified the PNPase-encoding gene (pnp) of Pseudomonas putida and constructed deletion mutants th...

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تجزیه ی حشره کش دیمتوات توسط باکتری Pseudomonas Putida

  Received: 21 April, 2009 Accepted: 8 July, 2009   Abstract   Background & Aims: Dimethoate as an organophosphorus compound is commonly used for crop production which is neurotoxic in human. Pseudomonas family harbor organophosphate degrading plasmids have been known as tools for cleaning environmental pollutions.   Materials & Methods: Pseudomonas Putida was isolated from contaminated soil b...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1972

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(19)44593-6